Cell-cycle-dependent Colonization of Mouse Spermatogonial Stem Cells After Transplantation into Seminiferous Tubules
نویسندگان
چکیده
منابع مشابه
Cell-cycle-dependent Colonization of Mouse Spermatogonial Stem Cells After Transplantation into Seminiferous Tubules
Spermatogonial stem cells (SSCs) migrate to the niche upon introduction into the seminiferous tubules of the testis of infertile animals. However, only 5-10% of the transplanted cells colonize recipient testes. In this study, we analyzed the impact of cell cycle on spermatogonial transplantation. We used fluorescent ubiquitination-based cell cycle indicator transgenic mice to examine the influe...
متن کاملP-103: Enhancement of Colonization on Mouse Spermatogonial Stem Cell by Low Intensity Ultrasound Stimulation
Background: Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis. New procedure such as sound wave especially low intensity ultrasound (LIUS) can be effective on increasing the number of cells. In this study we investigated the effect of LIUS stimulation on mouse SSCs. Materials and Methods: Isolated SSCs from neonate mice cultured in DMEM culture medium with 10% fetal bovine ...
متن کاملDifferentiation of human umbilical cord mesenchymal stem cells into germ-like cells in mouse seminiferous tubules
Our previous study demonstrated that human umbilical cord mesenchymal stem cells (HUMSCs) were capable of differentiation into germ cells in vitro. To assess this potential in vivo, HUMSCs were microinjected into the lumen of seminiferous tubules of immunocompetent mice, which were treated with busulfan to destroy endogenous spermatogenesis. Bromodeoxyuridine labeling studies demonstrated that ...
متن کاملFunctional sperm produced after spermatogonial stem cell transplantation into rhesus.
S permatogonial stem cell (SSC) transplantation is a promising technique to circumvent sterility in prepubertal boys undergoing gonadotoxic treatments. While the cryopreservation of spermatogonial stem cells is being introduced in clinical practices worldwide, a lot of unanswered questions remain regarding their eventual transplantation. In this paper autologous and allogeneic SSC transplantati...
متن کاملIncrease of Proliferation and Colonization in Mouse Spermatogonial Stem Cell by Low Intensity Ultrasound
Background Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis. Sound wave especially low intensity ultrasound (LIUS) can be effective on increasing the number of cells. In this study we investigated the effect of LIUS stimulation on mouse SSCs proliferation and colonization. Methods: Isolated SSCs from neonate mice cultured in DMEM culture medium with 10% Fetal Bovine S...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 2014
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.2013-083